Enzyme assembly on nanoparticle scaffolds enhances cofactor recycling and improves coupled reaction kinetics

Author:

Breger Joyce C.1ORCID,Goldman Ellen R.1ORCID,Susumu Kimihiro2ORCID,Oh Eunkeu2ORCID,Green Christopher M.1ORCID,Hooe Shelby L.13ORCID,Thakur Meghna14ORCID,Medintz Igor L.1ORCID,Ellis Gregory A.1ORCID

Affiliation:

1. Center for Bio/Molecular Science and Engineering Code 6900, U.S. Naval Research Laboratory, Washington, D.C., 20375, USA

2. Optical Sciences Division Code 5600, U.S. Naval Research Laboratory, Washington, D.C., 20375, USA

3. National Research Council, Washington, D.C., 20001, USA

4. College of Science, George Mason University, Fairfax, Virginia, 22030, USA

Abstract

We demonstrate that the phenomena of enzyme enhancement by nanoparticle display can be expanded to enzymatic cofactor recycling along with the coupled enzymatic processes that they participate in.

Funder

Strategic Environmental Research and Development Program

Office of Naval Research

National Institute of Food and Agriculture

U.S. Naval Research Laboratory

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

Reference93 articles.

1. National Research Council , Industrialization of Biology: A Roadmap to Accelerate the Advanced Manufacturing of Chemicals , The National Academies Press , Washington, DC , 2015

2. Systems biology based metabolic engineering for non-natural chemicals

3. Industrial biomanufacturing: The future of chemical production

4. Manufacturing Molecules Through Metabolic Engineering

5. Systems strategies for developing industrial microbial strains

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