Real-time <i>in situ</i> fluorescence imaging of telomerase and miR378 in living cells using a two-color DNA tetrahedron nanoprobe combined with molecular beacons-Reference-Cited by-同舟云学术

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Real-time in situ fluorescence imaging of telomerase and miR378 in living cells using a two-color DNA tetrahedron nanoprobe combined with molecular beacons

Published:2024 Issue:7 Volume:149 Page:2051-2058
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Guang Jiejie¹,Wang Shan¹,Fan Bingyuan¹,Yu Ziyao²,Gao Yahui¹,Pan Jinru¹,Xi Junting³,Meng Wei¹^ORCID,Hu Fang¹

Affiliation:

1. Key Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing 211198, China

2. Shanghai Asymchem Biotechnology Co., Ltd., Lane 795, Kangwei Road, J Pudong New District, Shanghai, China

3. School of Sciences, China Pharmaceutical University, Nanjing 211198, China

Abstract

A DNA tetrahedron nanoprobe (DTP) with a functionalized vertex and edge is designed for the reliable detection of miR378 and telomerase in living cells. This strategy is simple to prepare, highly specific, less cytotoxic, and biocompatible.

Funder

Natural Science Foundation of Jiangsu Province

Fundamental Research Funds for the Central Universities

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AN/D3AN02107F

Reference37 articles.

1. The functions of animal microRNAs

2. MicroRNAs

3. Oncomirs — microRNAs with a role in cancer

4. Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs

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