An integrated approach towards extracting structural characteristics of chlorosomes from a bchQ mutant of Chlorobaculum tepidum

Author:

Dsouza Lolita1ORCID,Li Xinmeng12ORCID,Erić Vesna3,Huijser Annemarie4ORCID,Jansen Thomas L. C.3ORCID,Holzwarth Alfred R.5ORCID,Buda Francesco1ORCID,Bryant Donald A.6ORCID,Bahri Salima7ORCID,Gupta Karthick Babu Sai Sankar1ORCID,Sevink G. J. Agur1ORCID,de Groot Huub J. M.1ORCID

Affiliation:

1. Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA, Leiden, The Netherlands

2. Department of Chemistry and Hylleraas Centre for Quantum Molecular Sciences, University of Oslo, 0315, Oslo, Norway

3. Zernike Institute of Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG, The Netherlands

4. MESA+ Institute for Nanotechnology, University of Twente, 7500 AE, The Netherlands

5. Max Planck Institute for Chemical Energy Conversion, Stiftstraße 34-36, 45470, Mülheim an der Ruhr, Germany

6. Department for Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA

7. NMR Spectroscopy, Bijvoet center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands

Abstract

The structure of the bchQ mutant of Cba. tepidum, with predominantly [8-Et, 12-Et]BChl c, is resolved using solid-state NMR, cryo-EM, and optical spectroscopy. Synanti parallel stacks run vertically with a subunit axial displacement of 1.49 nm.

Publisher

Royal Society of Chemistry (RSC)

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