A facile method for monitoring sphingomyelin synthase activity in HeLa cells using liquid chromatography/mass spectrometry-Reference-Cited by-同舟云学术

A facile method for monitoring sphingomyelin synthase activity in HeLa cells using liquid chromatography/mass spectrometry

Published:2024 Issue:12 Volume:149 Page:3293-3301
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Sundaraswamy Punith M.¹,Minami Yusuke²,Jayaprakash Jayashankar¹,B. Gowda Siddabasave Gowda¹³^ORCID,Takatsu Hiroyuki⁴,Gowda Divyavani³,Shin Hye-Won⁴,Hui Shu-Ping³

Affiliation:

1. Graduate School of Global Food Resources, Hokkaido University, Kita-9, Nishi-9, Kita-Ku, Sapporo 060-0809, Japan

2. Graduate School of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, Japan

3. Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, Japan

4. Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan

Abstract

An LC-MS/MS-based assay for monitoring sphingomyelin synthase (SMS) activity was developed. The method is highly useful in screening novel inhibitors for SMS.

Funder

Japan Society for the Promotion of Science

Japan Science and Technology Corporation

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AN/D4AN00304G

Reference33 articles.

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