Structural origin and rational development of bright red noncanonical variants of green fluorescent protein-Reference-Cited by-同舟云学术

Structural origin and rational development of bright red noncanonical variants of green fluorescent protein

Published:2023 Issue:23 Volume:25 Page:15624-15634
ISSN:1463-9076
Container-title:Physical Chemistry Chemical Physics
language:en
Short-container-title:Phys. Chem. Chem. Phys.

Author:

Chen Cheng¹^ORCID,Zhang Hao²³,Zhang Jing³,Ai Hui-wang²³⁴^ORCID,Fang Chong¹^ORCID

Affiliation:

1. Department of Chemistry, Oregon State University, 153 Gilbert Hall, Corvallis, Oregon 97331, USA

2. Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA

3. Department of Molecular Physiology and Biological Physics and Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA

4. The UVA Comprehensive Cancer Center, University of Virginia, Charlottesville, Virginia 22908, USA

Abstract

We used steady-state and time-resolved electronic and vibrational spectroscopies to reveal a “green” chromophore in a “red” protein with a single amino substituent, and rationally designed aY-sfGFP mutants with significantly brighter red emission.

Funder

National Institutes of Health

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Link

http://pubs.rsc.org/en/content/articlepdf/2023/CP/D3CP01315D

Reference74 articles.

1. Fluorescent proteins from nonbioluminescent Anthozoa species

2. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral