A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate-Reference-Cited by-同舟云学术

A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate

Published:2015 Issue:15 Volume:140 Page:5065-5073
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Obayashi Yusuke¹²³,Iino Ryota⁴⁵⁶³⁷,Noji Hiroyuki¹²³⁸⁹

Affiliation:

1. Department of Applied Chemistry

2. The University of Tokyo

3. Japan

4. Okazaki Institute for Integrative Bioscience

5. Institute of Molecular Science

6. National Institutes of Natural Sciences

7. SOKENDAI (The Graduate University for Advanced Studies)

8. CREST

9. Japan Science and Technology Agency

Abstract

Digitalization of fluorogenic enzymatic assays through the use of femtoliter chamber array technology is an emerging approach to realizing highly quantitative bioassays with single-molecule sensitivity.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/AN/C5AN00714C

Reference24 articles.

1. The Potential Impact of Droplet Microfluidics in Biology

2. Digital biology and chemistry

3. Digital, Ultrasensitive, End-Point Protein Measurements with Large Dynamic Range via Brownian Trapping with Drift

4. A Single Molecule Perspective on the Functional Diversity of in Vitro Evolved β-Glucuronidase

5. Distinct and Long-Lived Activity States of Single Enzyme Molecules

Cited by 47 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A Mem-dELISA platform for dual color and ultrasensitive digital detection of colocalized proteins on extracellular vesicles;2024-03-13

2. Functional analysis of single enzymes combining programmable molecular circuits with droplet-based microfluidics;Nature Nanotechnology;2024-02-26

3. Overview on the Development of Alkaline-Phosphatase-Linked Optical Immunoassays;Molecules;2023-09-11

4. Digital Cascade Assays for ADP- or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device;ACS Sensors;2023-08-17

5. Control of Reversible Formation and Dispersion of the Three Enzyme Networks Integrating DNA Computing;Analytical Chemistry;2023-05-30