Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks

Author:

Crowe J. A.1234ORCID,El-Tamer A.567,Nagel D.12348ORCID,Koroleva A. V.567910ORCID,Madrid-Wolff J.1112131415ORCID,Olarte O. E.1112131416ORCID,Sokolovsky S.172184,Estevez-Priego E.1920211422,Ludl A.-A.1920211422,Soriano J.1920211422ORCID,Loza-Alvarez P.11121314,Chichkov B. N.232425726ORCID,Hill E. J.1234ORCID,Parri H. R.1234ORCID,Rafailov E. U.172184ORCID

Affiliation:

1. School of Life and Health Sciences

2. Aston University

3. B4 7ET Birmingham

4. UK

5. Laser Zentrum Hannover e.V

6. D-30419 Hannover

7. Germany

8. Aston Medical School

9. Institute for Regenerative Medicine

10. Sechenov University

11. ICFO – Institut de Ciències Fotòniques

12. The Barcelona Institute of Science and Technology

13. 08860 Castelldefels

14. Spain

15. Universidad de los Andes

16. Universidad ECCI

17. Aston Institute of Photonic Technologies

18. Birmingham

19. Departament de Física de la Matèria Condensada

20. Universitat de Barcelona

21. 08028 Barcelona

22. Universitat de Barcelona Institute of Complex Systems (UBICS)

23. Institute of Quantum Optics

24. Leibniz University Hannover

25. 30167 Hannover

26. P.N. Lebedev Physical Institute of the Russian Academy of Sciences

Abstract

We identified photopolymers for 2-photon polymerisation with biocompatibility for human iPSC-derived neural network development. Generation of microscale scaffold topologies enabled neurite guidance, demonstrating use in reproducing aligned networks.

Funder

Horizon 2020 Framework Programme

Ministerio de Economía y Competitividad

Generalitat de Catalunya

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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