Enzymatic modification of 5′-capped RNA with a 4-vinylbenzyl group provides a platform for photoclick and inverse electron-demand Diels

Enzymatic modification of 5′-capped RNA with a 4-vinylbenzyl group provides a platform for photoclick and inverse electron-demand Diels–Alder reaction

Published:2015 Issue:2 Volume:6 Page:1362-1369
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Holstein Josephin Marie¹²³⁴,Stummer Daniela¹²³⁴⁵,Rentmeister Andrea¹²³⁴⁵

Affiliation:

1. Westfälische Wilhelms-Universität Münster

2. Institute of Biochemistry

3. 48149 Muenster

4. Germany

5. Cells-in-Motion Cluster of Excellence (EXC 1003 – CiM)

Abstract

Enzymatic transfer of 4-vinylbenzyl to the mRNA 5′-cap gives access to the fluorogenic photoclick and the inverse electron-demand Diels–Alder reaction.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/SC/C4SC03182B

Reference65 articles.

1. Selective Functionalization of a Genetically Encoded Alkene-Containing Protein via “Photoclick Chemistry” in Bacterial Cells

2. Live-Cell Imaging of Cellular Proteins by a Strain-Promoted Azide-Alkyne Cycloaddition

3. Enzymatic Site-Specific Functionalization of Protein Methyltransferase Substrates with Alkynes for Click Labeling