Evaluation of different direct and indirect SELEX monitoring methods and implementation of melt-curve analysis for rapid discrimination of variant aptamer sequences-Reference-Cited by-同舟云学术

Evaluation of different direct and indirect SELEX monitoring methods and implementation of melt-curve analysis for rapid discrimination of variant aptamer sequences

Published:2020 Issue:30 Volume:12 Page:3823-3835
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Mohammadinezhad Rezvan¹²³⁴,Jalali Seyed Amir Hossein¹²³⁴⁵^ORCID,Farahmand Hamid⁶⁷⁸⁹⁴

Affiliation:

1. Research Institute for Biotechnology and Bioengineering

2. Isfahan University of Technology

3. Isfahan

4. Iran

5. Department of Natural Resources

6. Department of Biotechnology

7. College of Agriculture and Natural Resources

8. University of Tehran

9. Karaj

Abstract

EMSA is the most cost-beneficial and accurate method for screening SELEX for protein targets and melt curve analysis in colony real-time PCR is a robust and effective method for distingsuishing variant aptamer sequences.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AY/D0AY00491J

Reference77 articles.

1. Advances in binder identification and characterisation: the case of oligonucleotide aptamers

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3. Aptamers and Their Biological Applications

4. Aptamer-Based Therapeutics: New Approaches to Combat Human Viral Diseases

5. Aptamers as targeted therapeutics: current potential and challenges

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