Comparison of sample preparation methodologies towards optimisation of Raman spectroscopy for peripheral blood mononuclear cells

Author:

Chaudhary Neha12345,Que Nguyen Thi Nguyet12345,Maguire Adrian12345,Wynne Claire6234,Meade Aidan D.12345ORCID

Affiliation:

1. School of Physics

2. Technological University Dublin

3. Dublin 8

4. Ireland

5. Radiation and Environmental Science Centre

6. School of Biological and Health Sciences

Abstract

Optimised protocols for treatment of peripheral blood monocytic cells for elimination of RBCs and separation of leukocyte components for Raman microspectroscopy.

Funder

Science Foundation Ireland

Health Research Board

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Reference47 articles.

1. Phenotypic and functional stability of leukocytes from human peripheral blood samples: considerations for the design of immunological studies

2. C. R. Kleiveland , Peripheral Blood Mononuclear Cells , in The Impact of Food Bioactives on Health: in vitro and ex vivo models , ed. Verhoeckx K. , Cotter P. , López-Expósito I. , Kleiveland C. , Lea T. , Mackie A. , et al. , Springer International Publishing , New York , 2015 , pp. 161–167

3. Perspectives on Systems Modeling of Human Peripheral Blood Mononuclear Cells

4. Integrated Raman and angular scattering microscopy reveals chemical and morphological differences between activated and nonactivated CD8+ T lymphocytes

5. Host responses from innate to adaptive immunity after vaccination: Molecular and cellular events

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