A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis-Reference-Cited by-同舟云学术

A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis

Published:2017 Issue:57 Volume:53 Page:7986-7989
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Lou Fei¹²³⁴⁵,Yang Jie¹²³⁴⁵,Wu Si¹²³⁴⁵,Perrett Sarah¹²³⁴⁵^ORCID

Affiliation:

1. National Laboratory of Biomacromolecules

2. CAS Center for Excellence in Biomacromolecules

3. Institute of Biophysics

4. Chinese Academy of Sciences

5. Beijing 100101

Abstract

Site-specific incorporation of donor–acceptor pair into a dimeric protein for single-molecule FRET analysis is realized based on a co-expression strategy.

Funder

Ministry of Science and Technology of the People's Republic of China

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2017/CC/C7CC03032K

Reference30 articles.

1. Chaperone Action at the Single-Molecule Level

2. Single-molecule FRET of protein structure and dynamics - a primer

3. A toolkit and benchmark study for FRET-restrained high-precision structural modeling

4. Three-dimensional molecular modeling with single molecule FRET

5. Identification of an Aggregation-Prone Structure of Tau

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