Controlled chemical assembly of enzymes in cell lysate enabled by genetic-encoded nonstandard amino acids

Author:

Zhang Jing1,Wang Ru2,Luo Zhiyuan1,Jia Dongmei1,Chen Haomin1,Xiao Qinjie1,Zhang Pengfei1,Pei Xiaolin1ORCID,Wang Anming1ORCID

Affiliation:

1. College of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Hangzhou Normal University, No. 2318, Road Yuhangtang, Hangzhou, Zhejiang 311121, China

2. School of Pharmacy, Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Hangzhou Normal University, No. 2318, Road Yuhangtang, Hangzhou, Zhejiang 311121, China

Abstract

Genetically encoded nonstandard amino acids (NSAAs) facilitate the covalent assembly of enzymes and enable their self-purification, enhanced activity and stability.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Zhejiang Province

National College Students Innovation and Entrepreneurship Training Program

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,General Materials Science

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