A comprehensive method to study the DNA's association with lamin and chromatin compaction in intact cell nuclei at super resolution-Reference-Cited by-同舟云学术

A comprehensive method to study the DNA's association with lamin and chromatin compaction in intact cell nuclei at super resolution

Published:2023 Issue:2 Volume:15 Page:742-756
ISSN:2040-3364
Container-title:Nanoscale
language:en
Short-container-title:Nanoscale

Author:

Chapman Katarina B.¹²^ORCID,Filipsky Filip³^ORCID,Peschke Nicolas¹^ORCID,Gelléri Márton²^ORCID,Weinhardt Venera⁴^ORCID,Braun Andrejs³^ORCID,Hausmann Michael¹^ORCID,Cremer Christoph¹²

Affiliation:

1. Kirchhoff-Institute for Physics, Heidelberg University, 69120 Heidelberg, Germany

2. Institute of Molecular Biology, Ackermannweg 4, 55128 Mainz, Germany

3. Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK

4. Centre for Organismal Studies, Heidelberg University, 69120 Heidelberg, Germany

Abstract

We expanded the fBALM (DNA structure fluctuation-assisted binding activated localization microscopy) method by developing a stable methodological sequence that enables dual-color imaging of high-resolution genomic DNA together with LaminB1.

Funder

Bundesministerium für Bildung und Forschung

Blood Cancer UK

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

Link

http://pubs.rsc.org/en/content/articlepdf/2023/NR/D2NR02684H

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