Aptamer selection against alpha-defensin human neutrophil peptide 1 on an integrated microfluidic system for diagnosis of periprosthetic joint infections-Reference-Cited by-同舟云学术

Aptamer selection against alpha-defensin human neutrophil peptide 1 on an integrated microfluidic system for diagnosis of periprosthetic joint infections

Published:2022 Issue:2 Volume:22 Page:250-261
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Gandotra Rishabh¹,Wu Hung-Bin²,Gopinathan Priya²^ORCID,Tsai Yi-Cheng²,Kuo Feng-Chih³^ORCID,Lee Mel S.⁴^ORCID,Lee Gwo-Bin¹²^ORCID

Affiliation:

1. Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan

2. Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan

3. Department of Orthopaedic Surgery, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan

4. Department of Orthopaedic Surgery, Paochien Hospital, Pingtung 90064, Taiwan

Abstract

An automated process was carried out on an integrated microfluidic system that featured SELEX 1) a positive + negative + competitive selection on a single chip and 2) aptamer-based ELISA-like assay for detection of PJI positive and negative samples.

Funder

Ministry of Science and Technology, Taiwan

National Health Research Institutes

Kaohsiung Chang Gung Memorial Hospital

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D1LC00969A

Reference49 articles.

1. Complications in Total Joint Arthroplasties