Hypothermic and cryogenic preservation of cardiac tissue-engineered constructs

Author:

Janssen Jasmijn1,Chirico Nino1,Ainsworth Madison J.2ORCID,Cedillo-Servin Gerardo2,Viola Martina23,Dokter Inge1,Vermonden Tina3ORCID,Doevendans Pieter A.45,Serra Margarida67,Voets Ilja K.8ORCID,Malda Jos29ORCID,Castilho Miguel10ORCID,van Laake Linda W.1,Sluijter Joost P. G.1,Sampaio-Pinto Vasco1,van Mil Alain1ORCID

Affiliation:

1. Department of Cardiology, Experimental Cardiology Laboratory, Circulatory Health Research Center, Regenerative Medicine Center Utrecht, University Utrecht, University Medical Center Utrecht, Uppsalalaan 8, Utrecht, 3584 CT, The Netherlands

2. Department of Orthopedics, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, 3584 CX, The Netherlands

3. Department of Pharmaceutical Sciences, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Universiteitsweg 99, 3508 TB Utrecht, The Netherlands

4. Netherlands Heart Institute (NLHI), Utrecht, 3511 EP, The Netherlands

5. Centraal Militair Hospitaal (CMH), Utrecht, 3584 EZ, The Netherlands

6. iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal

7. Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal

8. Laboratory of Self-Organizing Soft Matter, Department of Chemical Engineering and Chemistry & Institute of Complex Molecular Systems, Eindhoven University of Technology, Eindhoven 5600 MB, PO box 513, The Netherlands

9. Department of Equine Sciences, Faculty of Veterinary Sciences, Utrecht University, Yalelaan 1, Utrecht, 3584 CL, The Netherlands

10. Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, 5612 AE, The Netherlands

Abstract

This study is the first to preserve cardiac tissue engineered constructs with encapsulated iPSC-derived cardiomyocytes under hypothermic- and cryogenic conditions.

Funder

H2020 Marie Skłodowska-Curie Actions

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Technische Universiteit Eindhoven

Universiteit Utrecht

Universitair Medisch Centrum Utrecht

H2020 European Research Council

Netherlands Heart Institute

Publisher

Royal Society of Chemistry (RSC)

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