A fluorescent aptasensor with product-triggered amplification by exonuclease III digestion for highly sensitive ATP detection

Author:

He Shengnan12345,Qu Long678910,Tan Ying678910,Liu Feng678910,Wang Yu12345,Zhang Wei1011759,Cai Zhiming12345,Mou Lisha12345ORCID,Jiang Yuyang678910

Affiliation:

1. Shenzhen Xenotransplantation Medical Engineering Research and Development Center

2. Institute of Translational Medicine

3. Shenzhen Second People's Hospital

4. First Affiliated Hospital of Shenzhen University

5. Shenzhen

6. Department of Chemistry

7. Tsinghua University

8. Beijing

9. China

10. The Ministry-Province Jointly Constructed Base for State Key Lab – Shenzhen Key Laboratory of Chemical Biology

11. The Graduate School at Shenzhen

Abstract

An improved sensitivity of an ATP detection assay was achieved by using an allosteric probe and exonuclease III digestion product-triggered signal amplification.

Funder

National Natural Science Foundation of China

Guandong Natural Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

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