Enzyme-containing spin membranes for rapid digestion and characterization of single proteins-Reference-Cited by-同舟云学术

Enzyme-containing spin membranes for rapid digestion and characterization of single proteins

Published:2018 Issue:16 Volume:143 Page:3907-3917
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Liu Weijing¹²³⁴^ORCID,Pang Yongle⁵⁶⁷⁴^ORCID,Tan Hui-Yin¹²³⁴^ORCID,Patel Nitin⁸⁹¹⁰⁴,Jokhadze Gia⁸⁹¹⁰⁴^ORCID,Guthals Adrian¹¹⁹¹²⁴,Bruening Merlin L.¹²³⁴¹³^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry

2. University of Notre Dame

3. Notre Dame

4. USA

5. Department of Chemistry

6. Michigan State University

7. East Lansing

8. Takara Bio USA

9. Inc.

10. Mountain View

11. Mapp Biopharmaceutical

12. San Diego

13. Department of Chemical and Biomolecular Engineering

Abstract

Centrifugation of antibodies through enzyme-containing spin membranes yields large proteolytic peptides that enable confirmation of protein sequences and post-translational modifications.

Funder

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2018/AN/C8AN00969D

Reference56 articles.

1. Mass spectrometry-based proteomics: existing capabilities and future directions

2. Proteomics by Mass Spectrometry: Approaches, Advances, and Applications

3. Proteolytic enzyme-immobilization techniques for MS-based protein analysis