Label-free visualization of photosynthetic microbial biofilms using mid-infrared photothermal and autofluorescence imaging

Author:

Kato Ryo123ORCID,Maeda Kaisei4,Yano Taka-aki123,Tanaka Kan4,Tanaka Takuo123ORCID

Affiliation:

1. Institute of Post-LED Photonics, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima, Tokushima 770-0856, Japan

2. Innovative Photon Manipulation Research Team, RIKEN Center for Advanced Photonics, Wako, Saitama 351-0198, Japan

3. Metamaterials Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama 351-0198, Japan

4. Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Midori-Ku, Yokohama, Kanagawa 226-8503, Japan

Abstract

Photosynthetic microbial biofilms were studied using mid-infrared photothermal (MIP) microscopy in a label-free manner. The distribution of cyanobacterial cells and their extracellular polysaccharides in the biofilm matrix were successfully visualized.

Funder

Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering

Research Foundation for Opto-Science and Technology

University of Tokushima

Fusion Oriented REsearch for disruptive Science and Technology

Core Research for Evolutional Science and Technology

Japan Society for the Promotion of Science

ACT-X

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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