Rigidized 3-aminocoumarins as fluorescent probes for strongly acidic environments and rapid yeast vacuolar lumen staining: mechanism and application

Author:

Joniak Jakub1,Stankovičová Henrieta1ORCID,Budzák Šimon2ORCID,Sýkora Milan3,Gaplovská-Kyselá Katarína4ORCID,Filo Juraj1ORCID,Cigáň Marek1ORCID

Affiliation:

1. Department of Organic Chemistry, Faculty of Natural Sciences, Comenius University, 842 15, Bratislava, Slovakia

2. Department of Chemistry, Faculty of Natural Sciences, Matej Bel University, 974 01, Banská Bystrica, Slovakia

3. Laboratory for Advanced Materials, Faculty of Natural Sciences, Comenius University, 842 15, Bratislava, Slovakia

4. Department of Genetics, Faculty of Natural Sciences, Comenius University, 842 15, Bratislava, Slovakia

Abstract

Rigidized 3-aminocoumarins represent interesting yellowish-green-fluorescent turn-on pH probes under strongly acidic conditions and indicators capable of significantly improving yeast vacuolar lumen staining compared to the commercial CMAC derivates.

Funder

Ministerstvo školstva, vedy, výskumu a športu Slovenskej republiky

Horizon 2020 Framework Programme

Agentúra na Podporu Výskumu a Vývoja

European Regional Development Fund

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

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