A one-step immunoassay based on switching peptides for diagnosis of porcine epidemic diarrhea virus (PEDV) using screened Fv-antibodies

Author:

Kim Tae-Hun1,Park Jae-Yeon2,Jung Jaeyong1,Sung Jeong Soo1,Kwon Soonil1,Bae Hyung Eun1ORCID,Shin Hyun-Jin2,Kang Min-Jung3ORCID,Jose Joachim4ORCID,Pyun Jae-Chul1ORCID

Affiliation:

1. Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, Korea

2. College of Veterinary Medicine, Chungnam National University, Daejeon, 34134, South Korea

3. Korea Institute of Science and Technology (KIST), Seoul, Korea

4. Institute of Pharmaceutical and Medical Chemistry, Westfälischen Wilhelms-Universität Münster, Muenster, Germany

Abstract

In this study, a one-step immunoassay for porcine epidemic diarrhea virus (PEDV) based on Fv-antibodies and switching peptides was developed, and the assay results of PEDV were obtained by just mixing samples without any further reaction or washing steps.

Funder

National Research Foundation of Korea

Korea Health Industry Development Institute

Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry

Publisher

Royal Society of Chemistry (RSC)

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