High sensitivity (zeptomole) detection of BODIPY-labelled heparan sulfate (HS) disaccharides by ion-paired RP-HPLC and LIF detection enables analysis of HS from mosquito midguts-Reference-Cited by-同舟云学术

High sensitivity (zeptomole) detection of BODIPY-labelled heparan sulfate (HS) disaccharides by ion-paired RP-HPLC and LIF detection enables analysis of HS from mosquito midguts

Published:2023 Issue:11 Volume:15 Page:1461-1469
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Maciej-Hulme Marissa L.¹^ORCID,Leprince Anaëlle C. N.¹²,Lavin Andre³,Guimond Scott E.¹^ORCID,Turnbull Jeremy E.¹^ORCID,Pelletier Julien¹,Yates Edwin A.¹⁴^ORCID,Powell Andrew K.³^ORCID,Skidmore Mark A.¹⁴^ORCID

Affiliation:

1. Centre for Glycoscience Research and Training, School of Life Sciences, Keele University, Staffordshire, ST5 5BG, UK

2. Université de Rennes 1, Rue du Thabor, 35065 Rennes Cedex, France

3. School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK

4. Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK

Abstract

Ultra-sensitive disaccharide compositional analysis of heparin and heparan sulfate facilitated by ion-paired, reversed phase HPLC coupled with laser-induced fluorescence detection.

Funder

Biotechnology and Biological Sciences Research Council

Royal Society of Tropical Medicine and Hygiene

Engineering and Physical Sciences Research Council

Biochemical Society

Liverpool John Moores University

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AY/D2AY01803A