Fractionation and online mass spectrometry based on imaged capillary isoelectric focusing (icIEF) for characterizing charge heterogeneity of therapeutic antibody-Reference-Cited by-同舟云学术

Fractionation and online mass spectrometry based on imaged capillary isoelectric focusing (icIEF) for characterizing charge heterogeneity of therapeutic antibody

Published:2023 Issue:4 Volume:15 Page:411-418
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Kwok Teresa¹,Zhou Mike¹,Schaefer Anna¹,Bo Tao¹,Li Victor¹,Huang Tiemin¹,Chen Tong¹^ORCID

Affiliation:

1. Advanced Electrophoresis Solution Ltd, Cambridge, Canada

Abstract

A hybrid platform of icIEF fractionation and online icIEF-MS was developed for charge variant analysis after analytical icIEF-UV profiling, and successfully utilized for in-depth charge variant characterization of therapeutic antibodies.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AY/D2AY01670B

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