On-line determination by small angle X-ray scattering of the shape of hen egg white lysozyme immediately following elution from a hydrophobic interaction chromatography column-Reference-Cited by-同舟云学术

On-line determination by small angle X-ray scattering of the shape of hen egg white lysozyme immediately following elution from a hydrophobic interaction chromatography column

Published:2016 Issue:20 Volume:141 Page:5810-5814
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Kulsing Chadin¹²³⁴⁵,Komaromy Andras Z.¹²³⁴⁵,Boysen Reinhard I.¹²³⁴⁵^ORCID,Hearn Milton T. W.¹²³⁴⁵

Affiliation:

1. Australian Research Council Special Research Centre for Green Chemistry

2. Australian Centre for Research on Separation Science (ACROSS)

3. Monash University

4. Melbourne

5. Australia

Abstract

This study documents the use of an integrated approach, involving on-line HIC interfaced with SAXS measurements, to monitor the conformational status of proteins immediately upon elution from a chromatographic column.

Funder

Australian Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/AN/C6AN00851H

Reference25 articles.

1. H. P. Jennissen , in Methods Mol. Biol, Humana Press Inc., 2005, vol. 305, pp. 81–99

2. Mixed electrolytes in hydrophobic interaction chromatography†

3. Structures of multidomain proteins adsorbed on hydrophobic interaction chromatography surfaces