Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A

Author:

Omura Keita12345ORCID,Aiba Yuichiro12345ORCID,Onoda Hiroki12345ORCID,Stanfield Joshua Kyle12345ORCID,Ariyasu Shinya12345ORCID,Sugimoto Hiroshi67859ORCID,Shiro Yoshitsugu10115ORCID,Shoji Osami12345ORCID,Watanabe Yoshihito12345ORCID

Affiliation:

1. Department of Chemistry

2. Graduate School of Science

3. Nagoya University

4. Nagoya 464-0802

5. Japan

6. Core Research for Evolutional Science and Technology (CREST)

7. Japan Science and Technology Agency

8. Tokyo

9. RIKEN SPring-8 Center

10. Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamighori, Akoh

11. Hyogo

12. Research Center for Materials Science

Abstract

Mn-substituted full-length P450BM3 was constructed by transpeptidase Sortase A, showing catalytic hydroxylation of aliphatic C–H bonds with molecular oxygen.

Funder

Core Research for Evolutional Science and Technology

Japan Society for the Promotion of Science

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

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