Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase-Reference-Cited by-同舟云学术

Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase

Published:2022 Issue:2 Volume:3 Page:227-230
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Rodrigues Matthew J.¹²^ORCID,Giri Nitai³^ORCID,Royant Antoine⁴⁵,Zhang Yang⁶,Bolton Rachel¹²,Evans Gwyndaf²,Ealick Steve E.⁶^ORCID,Begley Tadhg³,Tews Ivo¹^ORCID

Affiliation:

1. Biological Sciences, Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK

2. Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK

3. Department of Chemistry, Texas A&M University, College Station, TX 77843, USA

4. Université Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale (IBS), CS 10090, Grenoble Cedex 9 38044, France

5. European Synchrotron Radiation Facility, CS 40220, Grenoble Cedex 9 38043, France

6. Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA

Abstract

Explaining stereoselective deprotonation and phosphate elimination in PLP biosynthesis through crystal structure, UV-vis absorption spectroscopic and mass spectrometric characterisation of a chromophoric intermediate.

Funder

Diamond Light Source

Welch Foundation

French Infrastructure for Integrated Structural Biology

Publisher

Royal Society of Chemistry (RSC)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Biology,Biochemistry,Chemistry (miscellaneous)

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CB/D1CB00160D

Reference21 articles.

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5. Structural Insights into the Mechanism of the PLP Synthase Holoenzyme from Thermotoga maritima,