Detection and identification of single ribonucleotide monophosphates using a dual in-plane nanopore sensor made in a thermoplastic via replication

Author:

Rathnayaka Chathurika12,Chandrosoma Indu A.12,Choi Junseo23ORCID,Childers Katie24,Chibuike Maximillian12ORCID,Akabirov Khurshed12,Shiri Farhad12,Hall Adam R.256ORCID,Lee Maxwell25,McKinney Collin7,Verber Matthew7,Park Sunggook23ORCID,Soper Steven A.12849ORCID

Affiliation:

1. Department of Chemistry, The University of Kansas, Lawrence, KS 66045, USA

2. Center of BioModular Multiscale Systems for Precision Medicine, USA

3. Mechanical & Industrial Engineering Department, Louisiana State University, Baton Rouge, LA 70803, USA

4. Bioengineering Program, The University of Kansas, Lawrence, KS 66045, USA

5. Virginia Tech-Wake Forest School of Biomedical Engineering and Sciences, Wake Forest School of Medicine, Winston Salem, NC 27101, USA

6. Atrium Wake Forest Baptist Comprehensive Cancer Center, Wake Forest School of Medicine, Winston Salem, NC 27157, USA

7. Department of Chemistry, University of North Carolina, Chapel Hill, Chapel Hill, NC 27599, USA

8. Department of Mechanical Engineering, The University of Kansas, Lawrence, KS 66045, USA

9. KU Cancer Center, University of Kansas Medical Center, Kansas City, KS 66160, USA

Abstract

We report the generation of ∼8 nm dual in-plane pores fabricated in a thermoplastic via nanoimprint lithography (NIL). This device was used to detect and identify single rNMP molecules via resistive pulse sensing.

Funder

National Institute of General Medical Sciences

National Institute of Biomedical Imaging and Bioengineering

National Cancer Institute

Publisher

Royal Society of Chemistry (RSC)

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