Allele specific DNAzyme assembly for fast and convenient SNP colorimetric genotyping directly from noninvasive crude samples-Reference-Cited by-同舟云学术

Allele specific DNAzyme assembly for fast and convenient SNP colorimetric genotyping directly from noninvasive crude samples

Published:2019 Issue:5 Volume:11 Page:596-603
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Huang Lizhen¹²³⁴⁵,Xia Qianfeng⁶⁷⁸⁹¹⁰,Zhang Yuhong¹²³⁴,Bai Huili¹²³⁴,Luo Nini¹²³⁴,Xiang Linguo¹²³⁴,Ding Shijia⁵¹¹¹²³⁴^ORCID,Cheng Wei¹²³⁴^ORCID

Affiliation:

1. The Center for Clinical Molecular Medical Detection

2. The First Affiliated Hospital of Chongqing Medical University

3. Chongqing 400016

4. P. R. China

5. Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education)

6. Laboratory of Tropical Biomedicine Technology

7. School of Tropical Medicine and Laboratory Medicine

8. Hainan Medical University

9. Haikou

10. China

11. College of Laboratory Medicine

12. Chongqing Medical University

Abstract

An allele specific DNAzyme assembly strategy was developed for fast and simple SNP genotyping directly from crude buccal swab samples without DNA extraction and purification.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/AY/C8AY02668H

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5. Diagnostic method validation: High resolution melting (HRM) of small amplicons genotyping for the most common variants in the MTHFR gene