Integrating error-prone PCR and DNA shuffling as an effective molecular evolution strategy for the production of α-ketoglutaric acid byl-amino acid deaminase-Reference-Cited by-同舟云学术

Integrating error-prone PCR and DNA shuffling as an effective molecular evolution strategy for the production of α-ketoglutaric acid byl-amino acid deaminase

Published:2016 Issue:52 Volume:6 Page:46149-46158
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Hossain Gazi Sakir¹²³⁴⁵,Shin Hyun-dong⁶⁷⁸⁹,Li Jianghua⁵¹⁰²³⁴,Wang Miao¹²³⁴,Du Guocheng⁵¹⁰²³⁴,Liu Long⁵¹⁰²³⁴,Chen Jian⁵¹⁰²³⁴

Affiliation:

1. School of Food Science and Technology

2. Jiangnan University

3. Wuxi 214122

4. China

5. Key Laboratory of Carbohydrate Chemistry and Biotechnology

6. School of Chemical and Biomolecular Engineering

7. Georgia Institute of Technology

8. Atlanta

9. USA

10. Ministry of Education

Abstract

l-Amino acid deaminases (LAADs; EC 1.4.3.2) belong to a family of amino acid dehydrogenases that catalyze the formation of α-keto acids froml-amino acids.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/RA/C6RA02940J

Reference49 articles.

1. Protein Design by Directed Evolution

2. Directed evolution of D-2-keto-3-deoxy-6-phosphogluconate aldolase to new variants for the efficient synthesis of D- and L-sugars

3. Stepwise error‐prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp

4. Dispelling the Myths--Biocatalysis in Industrial Synthesis

5. The production of fine chemicals by biotransformations

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