Passivating quantum dots against histag-displaying enzymes using blocking peptides: salient considerations for self-assembling quantum dot biosensors-Reference-Cited by-同舟云学术

Passivating quantum dots against histag-displaying enzymes using blocking peptides: salient considerations for self-assembling quantum dot biosensors

Published:2023 Issue:6 Volume:2 Page:1521-1530
ISSN:2635-0998
Container-title:Sensors & Diagnostics
language:en
Short-container-title:Sens. Diagn.

Author:

Green Christopher M.¹^ORCID,Hastman David A.¹²,Susumu Kimihiro³,Spangler Joseph¹,Stenger David A.¹,Medintz Igor L.¹^ORCID,Díaz Sebastián A.¹^ORCID

Affiliation:

1. Center for Bio/Molecular Science and Engineering Code 6900, U.S. Naval Research Laboratory, Washington, D.C. 20375, USA

2. American Society for Engineering Education, Washington, D.C. 20036, USA

3. Optical Sciences Division Code 5600, U.S. Naval Research Laboratory, Washington, D.C. 20375, USA

Abstract

Peptide-based passivation strategies were developed to prevent the unintended conjugation of his-tagged enzymes to quantum dot (QD) biosensors, enabling the application of QD biosensors in complex assays such as CRISPR-based diagnostics.

Funder

U.S. Naval Research Laboratory

Office of Naval Research

Institute for Nanoscience

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2023/SD/D3SD00149K

Reference62 articles.

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4. Effect of number of poly(His) tags on the adsorption of engineered proteins on immobilized metal affinity chromatography adsorbents

5. Cysteine and histidine shuffling: mixing and matching cysteine and histidine residues in zinc finger proteins to afford different folds and function

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