A continuous flow PCR array microfluidic chip applied for simultaneous amplification of target genes of periodontal pathogens

Author:

Yang Bo1,Wang Ping2,Li Zhenqing1ORCID,Tao Chunxian1,You Qingxiang1,Sekine Shinichi3ORCID,Zhuang Songlin1,Zhang Dawei1ORCID,Yamaguchi Yoshinori4

Affiliation:

1. Engineering Research Center of Optical Instrument and System, Key Lab of Optical Instruments and Equipment for Medical Engineering, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China

2. Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, China

3. Department of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Osaka 565-0871, Japan

4. Oono Joint Research laboratory, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan

Abstract

The concept of time to place conversion makes using a continuous flow polymerase chain reaction microfluidic chip an ideal way to reduce the time required for amplification of target genes; however, it also brings about low throughput amplicons.

Funder

Science and Technology Commission of Shanghai Municipality

University of Shanghai for Science and Technology

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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