High-contrast imaging of cellular non-repetitive drug-resistant genes <i>via in situ</i> dead Cas12a-labeled PCR-Reference-Cited by-同舟云学术

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High-contrast imaging of cellular non-repetitive drug-resistant genes via in situ dead Cas12a-labeled PCR

Published:2024 Issue: Volume: Page:
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Deng Ruijie¹^ORCID,Zhang Xinlei¹,Cao Jijuan²,Liu Xinmiao¹,Zhang Yong¹,Wang Feng³,Xia Xuhan¹

Affiliation:

1. College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China

2. Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, Liaoning 116600, China

3. Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, Guangdong, China

Abstract

We developed a dead Cas12a (dCas12a)-labeled polymerase chain reaction (CasPCR) assay for high-contrast imaging of cellular drug-resistant genes.

Funder

Natural Science Foundation of Sichuan Province

National Key Research and Development Program of China

Dalian Minzu University

Ministry of Education of the People's Republic of China

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CC/D4CC03059A

Reference30 articles.

1. Rolling circle amplification for single cell analysis and in situ sequencing

2. Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

3. Isothermal Amplification for MicroRNA Detection: From the Test Tube to the Cell

4. Wash-Free and High-Contrast Imaging of Single-Nucleotide Variation in Single Cells Using Transcription-Amplified Light-Up RNA Aptamer

5. Spatiotemporally resolved tools for analyzing gut microbiota

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