A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly-Reference-Cited by-同舟云学术

A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly

Published:2020 Issue:47 Volume:10 Page:28037-28040
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Liu Yang¹²³⁴^ORCID,Li Shihong¹²³⁴,Zhang Likun¹²³⁴,Zhao Qian¹²³⁴,Li Nuo¹²³⁴,Wu Yuxin¹²³⁴

Affiliation:

1. Department of Clinical Laboratory

2. The Third Affiliated Hospital of Jinzhou Medical University

3. Jinzhou

4. China

Abstract

We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR–Cas9 and catalytic hairpin assembly.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/RA/D0RA03603J

Reference25 articles.

1. miRNA: A Diagnostic and Therapeutic Tool for Pancreatic Cancer

2. MiRNA Biogenesis and Regulation of Diseases: An Overview

3. MicroRNAs in blood and cerebrospinal fluid as diagnostic biomarkers of multiple sclerosis and to monitor disease progression

4. Exosomes in perspective: a potential surrogate for stem cell therapy

5. Aptamer-Cholesterol-Mediated Proximity Ligation Assay for Accurate Identification of Exosomes

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