Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform-Reference-Cited by-同舟云学术

Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform

Published:2020 Issue:56 Volume:10 Page:34088-34098
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Ji Minghui¹²³⁴,Xia Yun⁵⁶⁷⁴,Loo Jacky Fong-Chuen⁸⁹⁴^ORCID,Li Lang¹⁰¹¹⁷⁴,Ho Ho-Pui⁸⁹⁴^ORCID,He Jianan⁵⁶⁷⁴,Gu Dayong¹²¹³¹⁴¹⁵⁷

Affiliation:

1. School of Nursing

2. Nanjing Medical University

3. Nanjing 211166

4. P. R. China

5. Shenzhen International Travel Health Care Center

6. Shenzhen Academy of Inspection and Quarantine

7. Shenzhen

8. Department of Biomedical Engineering

9. The Chinese University of Hong Kong

10. Shenzhen Bao'an Traditional Chinese Medicine Hospital (Group)

11. Guangzhou University of Chinese Medicine

12. Department of Laboratory Medicine

13. Shenzhen Second People's Hospital

14. The First Affiliated Hospital of Shenzhen University

15. Health Science Center

Abstract

Development of a microfluidic disc-direct reverse-transcription quantitative PCR platform to perform automated multiplex nucleic acid tests for rapid multiplex detection of disease infection.

Funder

Natural Science Foundation of Jiangsu Province

National Natural Science Foundation of China

National Basic Research Program of China

Guangdong Science and Technology Department

Sun Yat-sen University

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/RA/D0RA04507A

Reference57 articles.

1. The latest information of COVID-19 , August 12, 2020 , retrieved from: https://www.worldometers.info/coronavirus/

2. Rapid molecular diagnosis of infectious viruses in microfluidics using DNA hydrogel formation

3. Microfluidics-Based Enrichment and Whole-Genome Amplification Enable Strain-Level Resolution for Airway Metagenomics

4. The Global Influenza Strategy for 2019–2030 made by WHO provides a framework for countries and partners to approach influenza holistically , March 19, 2020 , retrieved from: https://apps.who.int/iris/bitstream/handle/10665/311184/9789241515320-eng.pdf

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