Switched reaction specificity in polyesterases towards amide bond hydrolysis by enzyme engineering

Author:

Biundo Antonino123,Subagia Raditya4523,Maurer Michael6783,Ribitsch Doris12345ORCID,Syrén Per-Olof910111213ORCID,Guebitz Georg M.12345

Affiliation:

1. Austrian Centre of Industrial Biotechnology (ACIB)

2. 3430 Tulln an der Donau

3. Austria

4. Institute of Environmental Biotechnology

5. University of Natural Resources and Life Sciences (BOKU)

6. Department of Bioengineering

7. University of Applied Sciences

8. 1140 Vienna

9. Science for Life Laboratory

10. Department of Fibre and Polymer Technology and Department of Protein Science

11. School of Engineering Sciences in Chemistry, Biotechnology and Health

12. KTH Royal Institute of Technology

13. 17165 Solna

Abstract

The constitution of a water network for the nitrogen inversion mechanism by H-bonding can increase amide-containing substrate acceptance of polyesterases.

Funder

Bundesministerium für Wissenschaft, Forschung und Wirtschaft

VINNOVA

Vetenskapsrådet

Svenska Forskningsrådet Formas

Kungliga Tekniska Högskolan

Standortagentur Tirol

Steirische Wirtschaftsförderungsgesellschaft

Österreichische Forschungsförderungsgesellschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

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5. J.Raynaud , J.Richens , A.Russell , United Nations Environment Programme and Plastic Disclosure Project. and Trucost (Firm) , Valuing plastic: the business case for measuring, managing and disclosing plastic use in the consumer goods industry , UNEP , 2014

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