Locus-specific detection of pseudouridine with CRISPR-Cas13a-Reference-Cited by-同舟云学术

Locus-specific detection of pseudouridine with CRISPR-Cas13a

Published:2024 Issue:30 Volume:60 Page:4088-4091
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Sun Mei¹,Fang Xin¹,Lin Bingqian¹^ORCID,Mo Jing¹^ORCID,Wang Fang²^ORCID,Zhou Xiang¹³^ORCID,Weng Xiaocheng¹^ORCID

Affiliation:

1. College of Chemistry and Molecular Sciences, Department of Clinical Laboratory of Zhongnan Hospital, Key Laboratory of Biomedical Polymers-Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China

2. Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China

3. Wuhan TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P. R. China

Abstract

A streamlined and efficient method enables precise detection of pseudouridine (Ψ) at specific loci, by leveraging CRISPR-Cas13a and CMC chemical labeling for immediate fluorescence analysis.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Fundamental Research Funds for the Central Universities

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CC/D4CC00179F

Reference22 articles.

1. Pseudouridine: the fifth RNA nucleotide with renewed interests

2. Dynamic RNA Modifications in Gene Expression Regulation

3. Transcriptome-wide Mapping Reveals Widespread Dynamic-Regulated Pseudouridylation of ncRNA and mRNA

4. Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells

5. Transcriptome-Wide Mapping of Pseudouridines: Pseudouridine Synthases Modify Specific mRNAs in S. cerevisiae

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