Phenotypic and transcriptional characterization of oligodendrocyte precursor cells in a 3D culture

Author:

Nakano Shizuka123,Uyeda Akiko1,Matsunaga Yukiko T.23ORCID,Muramatsu Rieko1

Affiliation:

1. Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and, Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502, Japan

2. Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan

3. Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

Abstract

Oligodendrocyte precursor cells (OPCs) showed phenotypic and transcriptomic differences between 2D culture and collagen-gel based 3D culture. OPCs cultured in a lower concentration of collagen gel enhanced their proliferation activity.

Funder

Japan Society for the Promotion of Science

Japan Agency for Medical Research and Development

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering

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