Nano-liter droplet libraries from a pipette: step emulsificator that stabilizes droplet volume against variation in flow rate
Author:
Affiliation:
1. Institute of Physical Chemistry
2. Polish Academy of Sciences
3. Warsaw
4. Poland
5. Institute of Theoretical Physics
Abstract
Many modern analytical assays, for example, droplet digital PCR, or screening of the properties of single cells or single mutated genes require splitting a liquid sample into a number of small (typically ca. nano-liter in volume) independent compartments or droplets.
Funder
European Research Council
Publisher
Royal Society of Chemistry (RSC)
Subject
Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering
Link
http://pubs.rsc.org/en/content/articlepdf/2016/LC/C6LC00265J
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5. One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution
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