Rapid, targeted and culture-free viral infectivity assay in drop-based microfluidics

Author:

Tao Ye12345,Rotem Assaf1234,Zhang Huidan12346,Chang Connie B.12347,Basu Anindita12348,Kolawole Abimbola O.910114,Koehler Stephan A.1234,Ren Yukun5121314,Lin Jeffrey S.1516174,Pipas James M.1819204,Feldman Andrew B.1516174,Wobus Christiane E.910114,Weitz David A.123421

Affiliation:

1. School of Engineering and Applied Sciences

2. Harvard University

3. Cambridge

4. USA

5. School of Mechatronics Engineering

6. Department of Cell Biology

7. Chemical and Biological Engineering Department

8. Broad Institute of MIT and Harvard 7 Cambridge Center

9. Department of Microbiology and Immunology

10. University of Michigan

11. Ann Arbor

12. Harbin Institute of Technology

13. Harbin 150001

14. China

15. Applied Physics Laboratory

16. Johns Hopkins University

17. Laurel

18. Department of Biological Sciences

19. University of Pittsburgh

20. Pittsburgh

21. Department of Physics

Abstract

We developed a rapid, targeted and culture-free infectivity assay using high-throughput drop-based microfluidics. The high sensitivity and large dynamic range of our cost effective assay alleviates the need for serial dilution experiments.

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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