Orthogonal protein decoration of DNA nanostructures based on SpyCatcher–SpyTag interaction-Reference-Cited by-同舟云学术

Orthogonal protein decoration of DNA nanostructures based on SpyCatcher–SpyTag interaction

Published:2022 Issue:97 Volume:58 Page:13471-13474
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Kröll Sandra¹^ORCID,Schneider Leonie¹^ORCID,Wadhwani Parvesh²^ORCID,Rabe Kersten S.¹^ORCID,Niemeyer Christof M.¹^ORCID

Affiliation:

1. Institute for Biological Interfaces (IBG 1), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Karlsruhe, Germany

2. Department of Molecular Biophysics (IBG 2), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Karlsruhe, Germany

Abstract

The SpyCatcher–SpyTag system (SC–ST) was used to efficiently couple sensitive ketoreductases to DNA origami, allowing for the study of their biocatalytic activity after immobilization.

Funder

Helmholtz-Gemeinschaft

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CC/D2CC05335G

Reference43 articles.

1. Folding DNA to create nanoscale shapes and patterns

2. DNA origami: a history and current perspective

3. Knitting complex weaves with DNA origami

4. DNA Origami: The Art of Folding DNA

5. Nucleic-Acid-Templated Enzyme Cascades

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