Azidophenyl as a click-transformable redox label of DNA suitable for electrochemical detection of DNA–protein interactions

Author:

Balintová Jana12345,Špaček Jan6785,Pohl Radek12345,Brázdová Marie6785,Havran Luděk67859,Fojta Miroslav67859,Hocek Michal12345

Affiliation:

1. Institute of Organic Chemistry and Biochemistry

2. Academy of Sciences of the Czech Republic

3. Gilead & IOCB Research Center

4. CZ-16610 Prague 6

5. Czech Republic

6. Institute of Biophysics

7. v.v.i. Academy of Sciences of the Czech Republic

8. 61265 Brno

9. Central European Institute of Technology

Abstract

A new azido-based DNA redox label which can be transformed into nitrophenyltriazole by a CuAAC click reaction was developed. It was used for the mapping of DNA–protein interactions with electrochemical detection.

Funder

Czech Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Reference69 articles.

1. E. Paleček , F.Jelen in Electrochemistry of nucleic acids and proteins: Towards electrochemical sensors for genomics and proteomics, ed. E. Paleček, F. Scheller, J. Wang, Elsevier, Amsterdam, 2005, pp. 74–174

2. J. Wang , in Electrochemistry of nucleic acids and proteins: Towards electrochemical sensors for genomics and proteomics, ed. E. Paleček, F. Scheller, J. Wang, Elsevier, Amsterdam, 2005, pp. 175–194

3. Electrochemistry of Nucleic Acids

4. Nucleobase modification as redox DNA labelling for electrochemical detection

5. Ferrocenylethynyl Derivatives of Nucleoside Triphosphates: Synthesis, Incorporation, Electrochemistry, and Bioanalytical Applications

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