Application of a fluorescently labeled trans-acting antigenomic HDV ribozyme to monitor antibiotic–RNA interactions-Reference-Cited by-同舟云学术

Application of a fluorescently labeled trans-acting antigenomic HDV ribozyme to monitor antibiotic–RNA interactions

Published:2015 Issue:24 Volume:7 Page:10414-10421
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Kasprowicz Aleksandra¹²³⁴,Kempińska Aleksandra¹²³⁴,Smólska Barbara¹²³⁴,Wrzesiński Jan¹²³⁴,Ciesiołka Jerzy¹²³⁴

Affiliation:

1. Institute of Bioorganic Chemistry

2. Polish Academy of Sciences

3. 61-704 Poznań

4. Poland

Abstract

In order to determine if a fluorescently labeled trans-acting antigenomic HDV ribozyme can be used to monitor antibiotic–RNA interactions, several parameters that can influence assay sensitivity and reproducibility were tested and optimized.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/AY/C5AY02953H

Reference37 articles.

1. Antibiotic discovery and development, ed. T. J. Dougherty and M. J. Pucci, Springer, New York, 2012

2. The Roles of RNA in the Synthesis of Protein

3. Ribosome-targeting antibiotics and mechanisms of bacterial resistance

4. The Impact of Aminoglycosides on the Dynamics of Translation Elongation

5. Crystal Structure of a Bioactive Pactamycin Analog Bound to the 30S Ribosomal Subunit

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