Development of a rapid pre-concentration protocol and a magnetic beads-based RNA extraction method for SARS-CoV-2 detection in raw municipal wastewater-Reference-Cited by-同舟云学术

Development of a rapid pre-concentration protocol and a magnetic beads-based RNA extraction method for SARS-CoV-2 detection in raw municipal wastewater

Published:2022 Issue:1 Volume:8 Page:47-61
ISSN:2053-1400
Container-title:Environmental Science: Water Research & Technology
language:en
Short-container-title:Environ. Sci.: Water Res. Technol.

Author:

Parra-Guardado A. L.¹^ORCID,Sweeney C. L.¹^ORCID,Hayes E. K.¹^ORCID,Trueman B. F.¹^ORCID,Huang Y.¹^ORCID,Jamieson R. C.¹^ORCID,Rand J. L.²^ORCID,Gagnon G. A.¹^ORCID,Stoddart A. K.¹^ORCID

Affiliation:

1. Centre for Water Resources Studies, Department of Civil & Resource Engineering, Dalhousie University, 1360 Barrington Street, Halifax, Nova Scotia, B3H 4R2, Canada

2. Acadia University, Ivan Curry School of Engineering, 15 University Avenue, Wolfville, Nova Scotia, B4P 2R6, Canada

Abstract

We demonstrate the application of a rapid pre-concentration protocol and a magnetic bead-based RNA extraction method for the detection of SARS-CoV-2 RNA from raw wastewater without the need for extensive sample treatment.

Funder

Natural Sciences and Engineering Research Council of Canada

Publisher

Royal Society of Chemistry (RSC)

Subject

Water Science and Technology,Environmental Engineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/EW/D1EW00539A

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