Chemo-enzymatic production of base-modified ATP analogues for polyadenylation of RNA-Reference-Cited by-同舟云学术

Chemo-enzymatic production of base-modified ATP analogues for polyadenylation of RNA

Published:2024 Issue:32 Volume:15 Page:13068-13073
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Mitton-Fry Rachel M.¹²^ORCID,Eschenbach Jannik¹^ORCID,Schepers Helena¹,Rasche René¹^ORCID,Erguven Mehmet¹³⁴^ORCID,Kümmel Daniel¹⁴^ORCID,Rentmeister Andrea¹³⁴^ORCID,Cornelissen Nicolas V.¹^ORCID

Affiliation:

1. Institute of Biochemistry, University of Münster, Corrensstr. 36, D-48149 Münster, Germany

2. Department of Chemistry and Biochemistry, Denison University, 100 W. College St., Granville, Ohio 43023, USA

3. Institute of Chemical Epigenetics, Ludwig-Maximilians-University Munich, Butenandtstr. 5-13, Haus F, D-81377 Munich, Germany

4. Cells in Motion Interfaculty Centre, University of Münster, Waldeyerstraße 15, D-48149 Münster, Germany

Abstract

We demonstrate the efficient chemo-enzymatic synthesis of base-modified adenosine-5′-triphosphate (ATP) analogues. These can be incorporated into RNA using poly(A) polymerase to produce RNAs with hypermodified poly(A) tails.

Funder

Deutsche Forschungsgemeinschaft

Denison University

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/SC/D4SC03769C

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