Light controlled reversible Michael addition of cysteine: a new tool for dynamic site-specific labeling of proteins-Reference-Cited by-同舟云学术

Light controlled reversible Michael addition of cysteine: a new tool for dynamic site-specific labeling of proteins

Published:2023 Issue:5 Volume:148 Page:1085-1092
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Maity Soham¹,Bingham Courtney¹,Sheng Wei²,Ehyaei Nona³,Chakraborty Debarshi¹,Tahmasebi-Nick Setare⁴,Kimmel Thomas E.¹,Vasileiou Chrysoula¹,Geiger James H.¹,Borhan Babak¹^ORCID

Affiliation:

1. Department of Chemistry, Michigan State University, 578 S. Shaw Ln., East Lansing, MI 48824, USA

2. Roche Tissue Diagnostics, 1910 E Innovation Park Dr, Oro Valley, AZ, 85755, USA

3. Lycia Therapeutics, 400 East Jamie Court, S San Francisco, CA 94080, USA

4. Merck & Co. Inc., 770 Sumneytown Pike, West Point, PA 19486, USA

Abstract

An engineered hCRBPII protein binds a coumarin-based fluorophore to yield a photoswitchable system via Michael/retro-Michael addition of a cysteine residue. Alternate UV/visible light irradiation accesses the two optical states.

Funder

U.S. Department of Energy

Michigan Economic Development Corporation

Michigan State University

National Institute of General Medical Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AN/D2AN01395A

Reference55 articles.

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3. Site-Specifically Labeled Immunoconjugates for Molecular Imaging—Part 1: Cysteine Residues and Glycans

4. Site-specific near-infrared fluorescent labelling of proteins on cysteine residues withmeso-chloro-substituted heptamethine cyanine dyes

5. Coumarin-Based Fluorogenic Probes for No-Wash Protein Labeling

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