Microfluidic label-free bioprocessing of human reticulocytes from erythroid culture

Author:

Zeming Kerwin Kwek123ORCID,Sato Yuko4567ORCID,Yin Lu89103,Huang Nai-Jia111213,Wong Lan Hiong1423,Loo Hooi Linn1423,Lim Ying Bena15163,Lim Chwee Teck151631716ORCID,Chen Jianzhu1819121320,Preiser Peter R.14232122ORCID,Han Jongyoon1232319ORCID

Affiliation:

1. Critical Analytics for Manufacturing of Personalized Medicine

2. Singapore-Massachusetts Institute of Technology Alliance for Research and Technology

3. Singapore

4. Department of Microbiology and Immunology

5. Keio University School of Medicine

6. Tokyo

7. Japan

8. Bioprocessing Technology Institute

9. Agency for Science

10. Technology and Research

11. Whitehead Institute for Biomedical Research

12. Cambridge

13. USA

14. Anti-Microbial Resistance

15. Department of Biomedical Engineering

16. National University of Singapore

17. Mechanobiology Institute

18. Department of Biology

19. Massachusetts Institute of Technology

20. The Koch Institute for Integrative Cancer Research

21. School of Biological Sciences

22. Nanyang Technological University

23. Department of Biological Engineering

Abstract

Developments in Dean flow fractionation (DFF) and deterministic lateral displacement (DLD) for label-free purification of cultured RBCs from human hematopoietic stem cells. An advancement in sorting and closed-loop manufacturing of viable human RBCs.

Funder

National Research Foundation Singapore

Singapore-MIT Alliance for Research and Technology Centre

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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