The critical role of Asp206 stabilizing residues on the catalytic mechanism of the <i>Ideonella sakaiensis</i> PETase-Reference-Cited by-同舟云学术

The critical role of Asp206 stabilizing residues on the catalytic mechanism of the Ideonella sakaiensis PETase

Published:2022 Issue:11 Volume:12 Page:3474-3483
ISSN:2044-4753
Container-title:Catalysis Science & Technology
language:en
Short-container-title:Catal. Sci. Technol.

Author:

Magalhães Rita P.¹²^ORCID,Fernandes Henrique S.¹²^ORCID,Sousa Sérgio F.¹²^ORCID

Affiliation:

1. UCIBIO – Applied Molecular Biosciences Unit, BioSIM – Departamento de Biomedicina, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal

2. Associate Laboratory i4HB – Institute for Health and Bioeconomy, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal

Abstract

We described the catalytic mechanism of IsPETase, a polyethylene-terephthalate degrading enzyme. The reaction was found to progress in four steps, divided in two events: formation of the first transition intermediate and hydrolysis of the adduct.

Funder

Fundação para a Ciência e a Tecnologia

Publisher

Royal Society of Chemistry (RSC)

Subject

Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CY/D1CY02271G

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