Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy-Reference-Cited by-同舟云学术

Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy

Published:2022 Issue:5 Volume:147 Page:784-788
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Jaiswal Mohit¹,Tran Trang T.¹,Guo Jiatong¹,Zhou Mingwei¹,Garcia Diaz Josefina¹,Fanucci Gail E.¹^ORCID,Guo Zhongwu¹^ORCID

Affiliation:

1. Department of Chemistry, University of Florida, 214 Leigh Hall, Gainesville, FL 32611, USA

Abstract

A new method is developed for spin labelling of cell surface sialoglycansviaenzymatic engineering to install azido-sialic acid and then click reaction to attach spin. EPR studies of labeled cells revealed the environments around the sialoglycans.

Funder

National Institute of General Medical Sciences

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AN/D1AN02226A

Reference36 articles.

1. Glycans in the immune system and The Altered Glycan Theory of Autoimmunity: A critical review

2. Density-dependent recognition of cell surface GM3 by a certain anti-melanoma antibody, and GM3 lactone as a possible immunogen: requirements for tumor-associated antigen and immunogen.