Analysis of cell–nanoparticle interactions and imaging of in vitro labeled cells showing barcorded endosomes using fluorescent thiol-organosilica nanoparticles surface-functionalized with polyethyleneimine

Author:

Nakamura Michihiro1ORCID,Nakamura Junna1,Mochizuki Chihiro1,Kuroda Chika1,Kato Shigeki1,Haruta Tomohiro2,Kakefuda Mayu2,Sato Shun3,Tamanoi Fuyuhiko45,Sugino Norihiro3

Affiliation:

1. Department of Organ Anatomy and Nanomedicine, Graduate School of Medicine, Yamaguchi University, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan

2. EM Application Group, EM Business Unit, JEOL Ltd., Japan

3. Department of Obstetrics and Gynecology, Graduate School of Medicine, Yamaguchi University, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan

4. Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA

5. Institute for Integrated Cell-Material Sciences, Institute for Advanced Study, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto 606-8501, Japan

Abstract

“Endosomal barcoded cells” via the differential intraendosomal sorting of particles depending on the particle surface were prepared using simultaneous dual-particle administration and observed on seamless biomedical imaging.

Funder

Japan Society for the Promotion of Science

Japan Science and Technology Agency

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Materials Science,General Chemistry,Atomic and Molecular Physics, and Optics,Bioengineering

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