Bacillus subtilis-based colorimetric bioassay for acute biotoxicity assessment of heavy metal ions-Reference-Cited by-同舟云学术

Bacillus subtilis-based colorimetric bioassay for acute biotoxicity assessment of heavy metal ions

Published:2015 Issue:73 Volume:5 Page:59472-59479
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Fang Deyu¹²³⁴⁵,Yu Yuan¹²³⁴⁵,Wu Liangzhuan¹²³⁴⁵,Wang Yu⁶⁷⁵,Zhang Jinghua⁶⁷⁵,Zhi Jinfang¹²³⁴⁵

Affiliation:

1. Key Laboratory of Photochemical Conversion and Optoelectronic Materials

2. Technical Institute of Physics and Chemistry

3. Chinese Academy of Sciences

4. Beijing 100190

5. PR China

6. Beijing Center for Physical & Chemical Analysis

7. Beijing 100089

Abstract

β-Galactosidase generated byBacillus subtiliscatalyzes the hydrolysis of ONPG to produce ONP, which can be detected at 420 nm and used to evaluate acute biotoxicity of heavy metal ions that inhibit the activity of the enzyme.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/RA/C5RA05452D

Reference51 articles.

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