cryoWriter: a blotting free cryo-EM preparation system with a climate jet and cover-slip injector-Reference-Cited by-同舟云学术

cryoWriter: a blotting free cryo-EM preparation system with a climate jet and cover-slip injector

Published:2022 Issue: Volume:240 Page:55-66
ISSN:1359-6640
Container-title:Faraday Discussions
language:en
Short-container-title:Faraday Discuss.

Author:

Rima Luca¹,Zimmermann Michael¹^ORCID,Fränkl Andri²,Clairfeuille Thomas³,Lauer Matthias³,Engel Andreas⁴,Engel Hans-Andreas⁴,Braun Thomas¹^ORCID

Affiliation:

1. Biozentrum, University of Basel, Spitalstrasse 41, 4056 Basel, Switzerland

2. Swiss Nanoscience Institute, University of Basel, 4056 Basel, Switzerland

3. Roche Pharma Research and Early Development, Therapeutic Modalities, Lead Discovery, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd., Grenzacherstrasse 124, 4070 Basel, Switzerland

4. cryoWrite Ltd., Klingelbergstrasse 50, 4056 Basel, Switzerland

Abstract

A microfluidic sample preparation method for TEM, which entirely avoids paper blotting and allows the passivation of the air–water interface during the preparation process, is presented.

Funder

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

Swiss Nanoscience Institute

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/FD/D2FD00066K

Reference34 articles.

1. Cryo-electron microscopy of vitrified specimens

2. Microscale Fluid Behavior during Cryo-EM Sample Blotting